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發(fā)布：admin 發(fā)布時間：2018-10-29 來源：本站蛋白激酶和磷酸酶對很多生物機(jī)制起著關(guān)鍵的調(diào)節(jié)作用，一般通過蛋白的磷酸化和去磷酸化調(diào)節(jié)諸如細(xì)胞生長、增殖、分化、代謝等生理過程，而細(xì)胞信號轉(zhuǎn)導(dǎo)中激酶和磷酸酶的活化異常將導(dǎo)致多種人類疾病。
目前，已經(jīng)發(fā)現(xiàn)人體內(nèi)含有超過600種激酶和磷酸酶，而快速篩選并開發(fā)針對這些酶類的潛在治療藥物，具有非常巨大的經(jīng)濟(jì)價值，F(xiàn)DA已經(jīng)批準(zhǔn)超過30種激酶類抑制劑藥物。
加拿大munechem公司新上市的廣譜?固相非放射性同位素生色反應(yīng)蛋白激酶活性測試盒， 可用于數(shù)十種重要的純化蛋白激酶的活性測試及其抑制劑的測試（用于高通量藥物篩選）
Broad Specific Kinase Assay Kit
| Catalog # ICP0248 |
Pack Size：96 assays
Product Description | This product is designed to detect the activity for variety of kinases (see Table 1) and screening the inhibitors of the corresponding kinase. The assay kit is non-radioactive but colorimetric. The principle of the assay is that: The kinase substrates is immobilized onto the 96-well plate. The kinases use ATP to phosphorylate the substrate on the plate. The phosphorylated product is detected by the phosphor-substrate specific antibodies. See figure1 for the principle illustration. | ||||||||||
Formulation | 1x96 assays or 5x96 assays | ||||||||||
Applications | Assay for the pure kinases listed in Table 1 Assay for inhibitors of the serine/threonine kinases listed in Table 1 | ||||||||||
Storage & Stability | Product is stable for 30 days at room remperature. 6 months at 4°C. | ||||||||||
Kit Compoentes
| 1. 96-well Substrate Microtiter Plate (Part#: KA101) 2. Phosphospecific Substrate Antibody(Part#: KA102) 3. Anti-Rabbit IgG: HRP Conjugate(Part#: KA103) 4. Antibody Dilution Buffer (Part#:KA104) 5. Kinase Assay Dilution Buffer (Part#:KA105) 6. ATP(Part#: KA106) 7. 20X Wash Buffer (Part#:KA107) 8. TMB Substrate (Part#:KA108) 9. Stop Solution (Part#:KA109) | ||||||||||
| Table 1: Kanise* activity tested by the Assay Kit | ||||||||
| No. | Kinase | Activity | No. | Kinase | Activity | No. | Kinase | Activity |
| 1 | AKT1 | +++++ | 32 | CLK2 | +++++ | 63 | PKAc beta | +++++ |
| 2 | AKT2 | +++++ | 33 | CLK3 | +++ | 64 | PKAc gamma | +++++ |
| 3 | AKT3 | +++++ | 34 | CLK4 | +++ | 65 | PKAc mu | +++++ |
| 4 | AMPK (A1/B1/G1) | +++++ | 35 | DAPK1 | ++++ | 66 | PKAc nu | +++++ |
| 5 | AMPK (A1/B1/G2) | +++++ | 36 | DAPK2 | ++++ | 67 | PKAc zeta | +++++ |
| 6 | AMPK (A1/B1/G3) | ++++ | 37 | DAPK3 | ++ | 68 | PKD2 | ++++ |
| 7 | AMPK (A1/B2/G1) | +++++ | 38 | DCAMKL2 | ++ | 69 | PKN2/PRK2 | ++++ |
| 8 | AMPK (A1/B2/G2) | +++++ | 39 | ERK5 | ++++ | 70 | PLK3 | +++ |
| 9 | AMPK (A1/B2/G3) | +++++ | 40 | KSR2 | +++++ | 71 | PLK4 (SAK) | +++ |
| 10 | AMPK (A2/B1/G1) | +++++ | 41 | MAK | ++++ | 72 | PRKG1 | +++++ |
| 11 | AMPK (A2/B1/G2) | +++++ | 42 | MAPKAPK2 | +++++ | 73 | PRKG2 | ++++ |
| 12 | AMPK (A2/B1/G3) | +++++ | 43 | MAPKAPK3 | ++++ | 74 | PRKX | +++++ |
| 13 | AMPK (A2/B2/G1) | +++++ | 44 | MAPKAPK5 | +++ | 75 | RIPK3 | ++++ |
| 14 | AMPK (A2/B2/G2) | ++++ | 45 | MARK1 | +++ | 76 | RSK1 | +++++ |
| 15 | AMPK (A2/B2/G3) | ++++ | 46 | MARK2 | ++++ | 77 | RSK2 | ++++ |
| 16 | AURORA A | +++++ | 47 | MARK3 | ++++ | 78 | RSK3 | ++++ |
| 17 | AURORA B | ++++ | 48 | MARK4 | ++ | 79 | RSK4 | +++ |
| 18 | AURORA C | ++++ | 49 | MELK | ++++ | 80 | SBK1 | +++++ |
| 19 | BMPR2 | +++ | 50 | MLK1 | +++ | 81 | SGK | +++ |
| 20 | CAMK1 beta | ++++ | 51 | MNK1 | ++++ | 82 | SGK2 | +++++ |
| 21 | CAMK1 delta | ++ | 52 | MNK2 | ++++ | 83 | SIK | ++++ |
| 22 | CAMK1 gamma | ++ | 53 | MSK1 | +++++ | 84 | SIK3 | ++++ |
| 23 | CAMK2 alpha | ++ | 54 | MYLK2 | +++ | 85 | STK39 (STLK3) | ++++ |
| 24 | CAMK2 beta | ++++ | 55 | Nuak2 (SNARK) | ++++ | 86 | TBK1 | +++ |
| 25 | CAMK2 delta | ++ | 56 | p70S6K | +++ | 87 | TSSK1B | ++++ |
| 26 | CAMK4 | ++++ | 57 | p70S6Kb | +++ | 88 | TSSK2 | +++++ |
| 27 | CDK6/CyclinD3 | ++++ | 58 | PAK3 | ++++ | 89 | ULK1 | +++ |
| 28 | CDK7/CyclinH1/MNAT1 | ++++ | 59 | PIM1 | ++ | 90 | ULK2 | +++ |
| 29 | CHK1 | +++++ | 60 | PIM2 | ++++ | 91 | ULK3 | ++++ |
| 30 | CHK2 | ++++ | 61 | PIM3 | ++ | 92 | WNK1 | ++ |
| 31 | CLK1 | +++++ | 62 | PKAc alpha | ++++ | |||